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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in several tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was located to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital job in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two extra splice variants have been described but detailed nucleotide sequence info just isn't nevertheless out there.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide typically consists of the epitope regarded with the antibody.
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This guideline outlines even more achievable optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This tutorial outlines additional achievable optimization of cellular and immunoassay parameters to be certain the best possible final results are...
Antibodies certain to get more info the blocking peptide no more bind to the epitope around the goal protein. By comparing the staining from your blocked antibody as opposed to the antibody by yourself, one can see which staining is distinct.
Focus on Information and facts Cyclin E belongs into the highly conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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